| abstract |
A method for accurately and easily detecting the production of microRNA (miRNA), and the diagnosis and treatment of diseases related to increase / decrease in microRNA-143 and 145, as well as the production of microRNA useful for treatment are adjusted. MicroRNA production regulators are provided. A method for detecting microRNA is described in which microRNA production is detected using a vector in which a microRNA binding site is created downstream of a luciferase gene and a recombinant cell into which the vector has been introduced. When microRNA binds to the microRNA binding site, translation of the luciferase gene is suppressed, the amount of luciferase expressed increases or decreases depending on the amount of generated microRNA, and the amount of microRNA is measured by measuring fluorescence intensity be able to. [Selection figure] None |