| abstract |
The present invention provides a method and device for spatially separating at least a first component and a second component in a sample, and in one exemplary embodiment, the first component and the second component are contained in a carrier fluid containing a spacer electrolyte solution. Introducing into a first microfluidic channel of a microfluidic device, stacking a first component and a second component between a leading electrolyte solution and a trailing electrolyte solution by isotachophoresis, the spacer electrolyte The solution comprises ions having a mobility in the electric field intermediate between the mobility of ions present in the leading electrolyte solution and the mobility of ions present in the trailing electrolyte solution, the spacer electrolyte solution comprising the following spacer ions: MOPS, MES, nonanoic acid, D-glucuronic acid, acetylsalicylic acid, 4- Toxibenzoic acid, glutaric acid, 3-phenylpropionic acid, phenoxyacetic acid, cysteine, hippuric acid, p-human oxyphenylacetic acid, isopropylmalonic acid, itaconic acid, citraconic acid, 3,5-dimethylbenzoic acid, 2,3-dimethyl Comprising at least one of benzoic acid, p-hydroxycinnamic acid, and 5-br-2,4-dihydroxybenzoic acid, wherein the first component comprises a DNA-antibody conjugate and the second component comprises a DNA-antibody Includes conjugates and analyte complexes. |