| abstract |
The present invention discloses a method for attaching and growing human corneal endothelial cultured cell monolayers on the substantial endothelium side synthesized from biopolymers to create a more biologically equivalent artificial cornea. This approach involves the use of adhesion and growth promoting substances such as fibronectin, laminin, RGDS, type IV collagen, bFGF bound to polycarbophil, and EGF bound to polycarbophil. The present application also provides a method for making an autonomous polymer comprising an adhesion molecule and a growth factor that supports the attachment and proliferation of human corneal endothelial culture cells for corneal transplantation as either a half-layer device or a full-layer button repalacement Is described. The present invention discloses an approach of transplanting cultured retinal pigment epithelium (RPE) cells into the subretinal space for the treatment of age-related macular degeneration (ARMD). This method makes it possible to deliver the transplanted RPE to the sheet of monolayer cells, which is more suitable for exerting its physiological function. |