http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2005507641-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 2002-02-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2005-03-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2005507641-A |
| titleOfInvention | Method for increasing heteroduplex complementarity |
| abstract | In vitro methods for increasing the complementarity of heteroduplex polynucleotides are described. The method uses reverse strand annealing to form duplexes of polynucleotides with mismatches. Heteroduplex polynucleotides have strand cleavage activity, 3 ′ → 5 ′ exonuclease activity, and polymerase activity, effective to give sufficient time for the percentage of complementarity to increase within the heteroduplex Combined with the amount of enzyme. Not all heteroduplex polynucleotides necessarily eliminate all mismatches complementarily. The resulting polynucleotide is optionally ligated. Multiple mutant polynucleotides are generated. At the site where each of the reverse strands is combined with the template to record the other strand, the result of the percentage complementarity of the heteropolynucleotide strand is increased. The parent polynucleotide need not be cleaved into fragments before annealing the heterologous strand. Therefore, there is no need for re-meeting. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2015509005-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018068311-A |
| priorityDate | 2001-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 235.