| abstract |
To provide a stratified culture method of epithelial cells without using feeder cells and a stratified epithelial cell sheet obtained therefrom. When epithelial cells are cultured on a porous membrane, both the upper layer and the lower layer are filled with the medium with the porous membrane as a boundary, and the medium is always passed through the porous membrane while the cells are cultured. Use a culture method that is supplied to cells from the lower layer. [Selection figure] None |