| abstract |
(57) [Summary] [Problem] To provide a method for producing a self-assembly with reduced reaction time, expansion of the optimal reaction temperature range, improved operability and applicability, and a simple method for detecting a gene using the method. I do. SOLUTION: For the formation of a plurality of pairs of dimers, the base region is composed of three regions of 3 'side, center and 5' side and the central region is complementary to each other and the 3 'and 5' side regions are non-complementary to each other. Using a probe and a plurality of pairs of cross-linking probes each comprising a 3′-side and a 5′-side region and comprising a non-complementary base sequence in the 3′-side and 5′-side regions, the cross-linking probe is subjected to the dimer formation. The dimer formed from the probe for use was formed into a base sequence capable of crosslinking, and the probe was hybridized so that the oligonucleotides self-assembled to form a double-stranded regular higher-order structure. |