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filingDate 2006-02-15-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af08f930eeeb9e7b5a2bcf21dee5aa45
publicationDate 2007-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber GB-2435326-A
titleOfInvention Heteroduplex analysis of non-human analytes
abstract Use of heteroduplex analysis for the taxonomic identification of a biological non-human analyte in a sample. Methods and kits for the taxonomic identification of non-human analytes, such as micro-organisms including viruses (eg. HIV), bacteria, fungi, or plants or foodstuffs, comprising obtaining a sample of a nucleic acid comprising a polymorphism from said non-human analyte, fragmenting said nucleic acid and combining the population of nucleic acid fragments with a synthetic nucleotide construct which forms duplexes with the nucleic acid fragments such that duplexes of different conformations are formed dependent upon the presence of the polymorphism and separating the duplexes to identify the taxomomic designation of the analyte. Alternatively claimed are synthetic DNA constructs for use in induced heteroduplex generator (IHG) analysis which correspond to a known polymorphic site in a genomic DNA which comprise either (i) the wild-type and the mutant base or (ii) neither the wild type or mutant base.
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