http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-3971169-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9ac656a6e2f3adc6c1b2d1276422116a |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-501 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y601-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D257-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B57-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43504 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D257-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C09B57-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-198 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D257-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D257-08 |
| filingDate | 2016-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78513c7b140baf8175ffe02570984a1b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f1b77f73323c46ebaab15eb669242de |
| publicationDate | 2022-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-3971169-A1 |
| titleOfInvention | Method for producing, in a cell, a protein or a polypeptide comprising a tetrazine amino acid |
| abstract | The present patent application refers to: n 1. A method for producing, in a cell, a protein or a polypeptide comprising at least one tetrazine amino acid, comprising: n • culturing a cell that comprises a nucleic acid that comprises at least one selector codon and encodes the protein or polypeptide in a medium including a tetrazine amino acid, wherein the cell includes an orthogonal tRNA that functions in the cell and recognizes the selector codon and an orthogonal aminoacyl tRNA synthetase, and wherein the orthogonal aminoacyl tRNA synthetase preferentially aminoacylates the orthogonal tRNA with the tetrazine amino acid; and n• in the cultured cell, incorporating the tetrazine amino acid into the protein or polypeptide to produce the protein or polypeptide comprising the at least one tetrazine amino acid residue. n 2. A method for making a cell with translation components, the method comprising: n • manufacturing in a cell, an orthogonal tRNA and an orthogonal aminoacyl tRNA synthetase, wherein the orthogonal tRNA recognizes the orthogonal aminoacyl tRNA synthetase, and wherein the orthogonal aminoacyl tRNA synthetase preferentially aminoacylates the orthogonal tRNA with a tetrazine amino acid. n 3. A kit, comprising: n • a cultured cell that comprises a nucleic acid in a medium including a tetrazine amino acid, wherein the cell includes an orthogonal tRNA; and n• an orthogonal aminoacyl tRNA synthetase, and wherein the orthogonal aminoacyl tRNA synthetase preferentially aminoacylates the orthogonal tRNA with the tetrazine amino acid. |
| priorityDate | 2015-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
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