| abstract |
The present invention relates to new methods and reagents for coupling molecules by a so-called click reaction in the presence of a suitable catalyst and divalent metal cations. Further, the invention relates to an activator composition for such click ligation reaction, a click ligation reagent kit, a device for performing such click ligation reaction, and the use of such method, composition, reagent kit and device to improve the efficiency of coupling of molecules via a click reaction, especially in the context of next generation nucleic acid sequencing methods. |