http://rdf.ncbi.nlm.nih.gov/pubchem/patent/AU-2020410970-B2
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_473c5c9b53e303173b1374acf4160ca9 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07049 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-131 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-155 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-109 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-191 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2535-122 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6855 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6855 |
filingDate | 2020-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2022-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59527182161ca0b6b4ada7a25505974c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dba7ef2214f6c393123869407fe1eaee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_267f4ee19942615f4efed39d8b8b03a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c81935e5fc4f06600bffaa5eda5cf1e |
publicationDate | 2022-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | AU-2020410970-B2 |
titleOfInvention | Method of amplifying mRNAs and for preparing full length mRNA libraries |
abstract | An inventive method for amplifying at least one RNA which is contained in a sample includes reverse transcribing the at least one RNA using a first primer, adding a dideoxy nucleotide which is modified in the 3'-position with a first partner of a pair of azide and alkyne molecules by action of a template independent polymerase to attach a single 3'-azide- or 3'-alkyne-modified dideoxy nucleotide at the 3'-end of the obtained cDNA, adding an adapter molecule which comprises a polynucleotide sequence and at its 5'-end a second partner of such pair of azide and alkyne molecules and ligating the adapter to the 3'-modified cDNA under formation of a triazole linkage, adding a second primer which is complementary to at least a part of the adapter molecule and which contains at its 3'-end a nucleotide which is complementary to the dideoxy nucleotide at the 3'-end of the cDNA to effect hybridization and binding of the second primer overlapping the triazole linkage, adding a third primer and amplifying the full length cDNA. Variations of this method are also disclosed. Uses of such method especially for preparing a full length RNA library and for sequencing of a plurality of RNAs contained in a sample, as well as reagent kits for perfoming such methods are also disclosed and included in the invention. |
priorityDate | 2019-12-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 273.