| abstract |
The present invention provides a method for obtaining biopharmaceuticals from transgenic bovine animals, where cDNA sequences of either human growth hormone, human insulin or human erythropoietin have been inserted into the genome of a transgenic bovine using a pMilk expression vector. The present invention relates to a method comprising the steps of using transgenic cell lines for generating transgenic cloned embryos, wherein the primary cell lines grown from fetal bovine skin tissue serve as donor cells, inserting into the selected cells of primary embryonic fibroblast cell line a pMILK plasmid into which, after a β-casein promoter, cDNA sequences encoding human insulin, growth hormone or erythropoietin have been cloned, using a neomycin (G418) resistance test for selecting transgenic cells and cloning the transgenic cell lines, implanting the cloned embryos, and achieving the birth of a healthy transgenic calf whose genome contains the genes of erythropoietin, growth hormone or insulin. |