http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2365337-A1

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filingDate 2011-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2011-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-2365337-A1
titleOfInvention Intracellular pH imaging method and apparatus using fluorescence lifetime
abstract The pH of a substance to be measured can be measured without being influenced by the intensity of excitation light (L1), the concentration of fluorescent material and surrounding environments. In a pH measurement method, pulsed excitation light (L1) including a wavelength that can excite a predetermined fluorescent material (P) contained in living matter is generated. The fluorescent material acts as coenzyme in oxidation/reduction reaction in vivo. Further, the intensity of the pulsed excitation light (L1) does not damage a tissue nor a cell in the living matter, and does not substantially change the pH of the living matter. Further, a predetermined position in the living matter is illuminated with the pulsed excitation light (L1), and light including fluorescence emitted from the fluorescent material excited by illumination with the pulsed excitation light (L1) is received. The lifetime (Tf) of the fluorescence is calculated by time-resolving the intensity of the received fluorescence, and the pH of the living matter is measured based on the lifetime (Tf).
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