| abstract |
The present invention provides a production method of a functional recombinant human hemoglobin, which comprises transforming a yeast of the genus Pichia with an expression vector containing a DNA encoding a human hemoglobin α-chain (Hbα), which is under regulation of a promoter functional in a yeast of the genus Pichia, and an expression vector containing a DNA encoding a human hemoglobin β-chain (Hbβ), which is under regulation of a promoter functional in a yeast of the genus Pichia, culturing the yeast in a medium, and recovering the human hemoglobin from the obtained culture. The present invention also provides an expression vector containing AOX1 promoter, DNA encoding Hbβ, terminator functional in a yeast of the genus Pichia, AOX1 promoter, DNA encoding Hbα and terminator functional in the yeast of the genus Pichia in this order from the upstream side in transcriptional direction. |