| abstract |
A device for a detection of a nucleotide sequence of an organism, said device comprising: n- at least one amplification chamber (1) for an amplification of the nucleotide sequence into amplified target nucleotide sequences, the said chamber (1) having an inlet port (11) for an introduction into the amplification chamber of an amplification solution (1) comprising said nucleotide sequence and reagents for nucleotide sequence amplification, having a first outlet channel (13) and is made of solid material resistant to at least 90°C; n- at least one reagent chamber (2), said reagent chamber (2) having a second inlet port (12) for introduction of the reagent and a second outlet channel (14); n- a detection chamber (3) having fixed upon one of its solid support surface (15) at least one capture nucleotide probe being immobilized in discrete regions of said surface (15) to form a micro-array (16), said capture nucleotide probe being capable to bind by complementary base pairing with the amplified target nucleotide sequence or fragments thereof, wherein said surface (15) of the detection chamber (3) having fixed the capture nucleotide probe is maintained flat at temperature higher than 50°C, wherein the flatness tolerance of said surface (15) is less than 800 nm, when heated at 50°C and wherein said surface (15) has a light transmittance higher than 60% at wavelength between 400 and 600 nm and, n- a fragmentation chamber (4) for a fragmentation of amplified target nucleotide sequences into fragments, the said fragmentation chamber being connected to the amplification chamber (1) and to the reagent chamber (2) by the first and second outlet channels (13, 14) and being connected to the detection chamber (3) by a third outlet channel (19) and wherein said first and second channels (13, 14) are located at a different level than the third channel (19). |