| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_785ac2b35073f5a6d251678e6fb31b6f |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K-
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2004-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_152fb2ac6ba76240d9cecd6cec1432b4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d49f7b0ecc5d56e39018e20bdeeb1948
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79f0d6108d32e9ba2766f08c545aae4f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_19b52e0df1e1a2079ff692886e52d7a1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ce9b00d5493824ed21b63656c501426 |
| publicationDate |
2006-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-1660512-A2 |
| titleOfInvention |
Oligonucleotide synthesis with alternative solvents |
| abstract |
The invention provides for methods of manufacturing an oligonucleotide comprising a pentavalent phosphate triester. In particular, the method comprises providing a 5' blocked-nucleoside, deblocking the 5' blocked-nucleoside to form a 5' OH-nucleoside, coupling the 5' OH-nucleoside with a phosphoramidite to form and oligonucleotide comprising a trivalent phosphite triester; and oxidizing the oligonucleotide comprising a trivalent phosphite triester to the oligonucleotide comprising a pentavalent phosphate triester. In some embodiments, the wash between any of the steps above is with at least one solvent wash comprising a toluene. |
| priorityDate |
2003-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |