| abstract |
The present invention provides a process for determining the quantitative andnqualitative profile of the repertoire of a given type of an immunoglobulin (Ig)nheavy chain expressed by a lymphocyte B population present in a tissue sample,nand kits and uses thereof. It also provides a set of VH forward primersnassociated with a CH reverse primer, which are respectively capable ofnspecifically hybridizing in stringent conditions with the nucleic acids encodingnthe variable segments of Ig heavy chains and with the constant segment of angiven type of Ig heavy chain, such as preferably IgM, IgG, IgE or IgA heavynchain. Methods for the in vitro diagnosis of a condition associated with annabnormal expression of the repertoire of a given type of Ig heavy chain, and fornthe in vitro follow-up of a treatment of such a condition, are also comprisednherein. |