http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0881293-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f1144e66b5feb529e22d400c1e870f87 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07C59-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-625 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P7-42 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-42 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P7-62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C59-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 |
| filingDate | 1997-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 1998-12-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EP-0881293-A1 |
| titleOfInvention | Production of medium chain length poly-3-hydroxy alkanoates in Escherichia coli, and monomers derived therefrom |
| abstract | Production of medium chain length poly-3-hydroxyalkanoatesn(mcl-PHAs) in Escherichia coli is reported for the firstntime. To produce PHA in E . coli , a PHA polymerase encodingngene ( phaC2 ) of Pseudomonas oleovorans GPo1 was introducedninto various E . coli strains and fatty acid oxidation mutantsnthereof. Of these, E . coli fadA/B , E . coli fadRfadA or E.n coli fadRfadB mutants, which are defective in specific stepsnof the fatty acid β-oxidation pathway, allowed production ofnmcl-PHAs from fatty acids ranging from octanoate to octadecanoate.nMaximum amounts of about 10% PHA of total cell drynweight (w/w) were found. The predominant monomers found innthese PHAs were always the same, independently of the chainnlength of the supplied fatty acids: 3-hydroxyoctanoate forneven chain length fatty acids and a combination of 3-hydroxynonanoatenand 3-hydroxyheptanoate for odd chain length fattynacids. Since the monomer composition of the produced polymernwas independent of the fatty acid chain length, β-oxidationnoccurs in these β-oxidation mutants. The polymer may benhydrolyzed to yield interesting optically active monomers andntheir esters. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20210027212-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1118629-A3 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1118629-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116445440-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100447532-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2510037-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2510037-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008135065-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2004033671-A1 |
| priorityDate | 1997-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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