| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7ad58d3c0b071069a8ee0cb8c8ba2e8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-966
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2334-70 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-26
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-54
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 |
| filingDate |
1997-05-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23ede72f79641fe1e12bdddd6199def8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3fb2b31028fa1bb90dbd2780b80ede62 |
| publicationDate |
1997-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0810290-A2 |
| titleOfInvention |
Method for end pointing enzyme detection methods |
| abstract |
The present invention involves a composition and method for the determination of enzymatic activity or enzyme substrate concentration in a fluid test sample in which the enzyme catalyzes a reaction in a reagent system to provide a detectable response indicative of the concentration of the enzyme or enzyme substrate in the test sample. A measured amount of a protease capable of eliminating the enzymatic activity is included in the reagent composition, so that when a measured amount of test sample is treated, the reaction producing the detectable response will have a definite end point. The invention is particularly useful in conjunction with the determination of leucocytes in urine wherein the leukocytes are lysed to release their human leucocyte elastase which hydrolyzes a chromogenic ester to provide a phenol which then reacts with a diazonium salt to form a colored azo dye. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2010059068-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03040405-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8460863-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8198073-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8206944-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03046211-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8741593-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2831889-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2831888-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9951768-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2832729-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7238483-B2 |
| priorityDate |
1996-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |