| abstract |
The present invention provides a method of assaying a sample of blood or blood components, in particular serum, for the presence of 1,25-dihydroxy-vitamin D comprising removing proteins from the sample, incubating the protein-free sample with a salt of periodic acid, isolating 1,25-dihydroxy-vitamin D from the periodate-treated sample, and determining the concentration of 1,25-dihydroxy-vitamin D. |