| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f2253d4109af8824a438cb92468bbda0 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
1992-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d87005d0efa529c651160d592cc5af0
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6ef3c61ea9896596fdbbb32b3b6cb4d |
| publicationDate |
1993-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
EP-0522884-A1 |
| titleOfInvention |
Process for controlling contamination of nucleic acid amplification reactions |
| abstract |
A method of incorporating an exo-sample nucleotide (e.g. deoxyuridine in DNA) into amplified product strands resulting from a nucleic acid amplification process is disclosed. Once the product strands have been obtained and analysed (e.g. by hybridisation, Southern blot, etc.), the exo-sample strands can be selectively destroyed by acting on the incorporated exo-sample nucleotide. n Two procedures are described. In the first procedure the exo-sample nucleotide is incorporated by conducting the amplification reaction in the presence of an excess of exo-sample nucleotide triphosphate. n In the second procedure the exo-sample nucleotide is incorporated by conducting the amplification reaction in the presence of an oligonucleotide which has, as part of its sequence, one or more exo-sample nucleotides. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11466311-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9602672-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5605796-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0624643-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0624643-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0897990-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0709468-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113293200-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0897990-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5763186-A |
| priorityDate |
1991-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |