| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c77702eca4fa8f5bdcf34a3276b07267 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate |
2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e40427c6ff778926e3cecca302f7dcd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a069da7e51fa02a503731dd190b08e3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc8db377c6f4fd0d3f77e12c22b91c41
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ecece051de36ec7d5a312a55da533817
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_adb7116cbf4186aaa4c8f322d74e9061 |
| publicationDate |
2021-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-113293200-A |
| titleOfInvention |
A method and application for reducing or eliminating the contamination of amplification products in second-generation sequencing |
| abstract |
The present disclosure provides a method for reducing or eliminating nucleic acid contamination of amplification products during second-generation sequencing and application in the detection of novel coronavirus 2019-nCoV. Aiming at the common false positive problem of next-generation sequencing platforms, the library is constructed through the long-range amplification-amplicon fragmentation scheme, and dUTP and nucleases that can specifically recognize dUTP are used in the long-range amplification system. The method of the present disclosure can reduce or eliminate false positives caused by contamination of amplification products, and improve the accuracy of detection. |
| priorityDate |
2021-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |