| abstract |
Novel signal peptides capable of functioning in yeasts have the amino acid sequence: nMet-A₁-A₂-X-B-C-D-E-F nwherein A₁ is a peptide chain composed of 1-3 amino acids each selected from the group consisting of Arg, Ser, Lys and His, A₂ is a peptide chain composed of 1-3 amino acids, X is a peptide chain composed of 8-10 hydrophobic amino acids, B is Pro or Ser, C is Gly or Pro, D Cys, Ala, Leu, Ser, Thr and Val, E is Trp or Gln and F is Ala or Gly. DNA sequences coding for these signal peptides can be used for the secretory expression of heterologous proteins in yeasts. |