| abstract |
Compounds of the general formula I are claimed in the Z is an organic or inorganic acid residue or a sugar residue, A an organic or inorganic acid residue, B an organic acid residue and R² and R⁵, which may be the same or different, are hydrogen, halogen or a lower alkyl group, R¹, R³, R⁴ and R⁶, which may be the same or different, are hydrogen, halogen, a cyano, lower alkyl, lower alkoxy, carboxy, lower alkoxycarbonyl, carboxy lower alkyl, lower alkoxycarbonyl lower alkyl or an optionally mono- or disubstituted Carboxamido group or the group -COO- (CH₂CH₂O) n -R⁷ in the R⁷ is hydrogen or a lower alkyl group and n is an integer from 1 to 4, where R⁶ can also represent a sulfo or nitro group, and a process for their preparation. n n n The compounds according to the invention are particularly suitable as substrates for determining the activity of hydrolases.n n n Another object of the application is a reagent for the determination of hydrolases, which contains an oxidizing agent and one or more compounds of the general formula I as chromogenic and fluorogenic substrate. |