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filingDate 1987-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1988-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber EP-0273127-A2
titleOfInvention Method to produce metabolites in eucaryotic cells using gene technology
abstract For the genetic engineering production of metabolic products in eukaryotic cells by introducing a foreign gene encoding the desired metabolic product using a retroviral provirus as a vector into a eukaryotic cell and expression of the product encoded by the foreign gene, the foreign gene to be expressed is built into a provirus integrated in a vector, the no longer contains functional genes for the replication and packaging functions gag, pol or / and env, introduces the vector thus obtained, which contains the provirus, and a further, at least one marker function, but no vector containing genes interfering with the packaging functions into a eukaryotic cell , which contains the retroviral genes of the replication and packaging functions gag, pol or / and env in the genome, with provirus and retroviral genes together in the genome having to encode a complete retrovirus, clones the transfected cell and selects by brand Function and desired metabolic product, infects a eukaryotic cell with the retroviruses formed in the growth medium of the selected cells and obtains the desired metabolic product from the infected cell or its growth medium.
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