| abstract |
Nucleotide derivatives useful in modified DNA probe synthesis are compounds having the formula (I) wherein either A is a radical of formula -NHCO-(CH₂) x -R³ and B is a substituted pyrimidine or purine residue, or A is a hydrogen atom and B is a radical of formula (II)n The terminal group R³ may comprise amino or carboxy groups, or a sugar moiety, or it may comprise a reporter group such as biotin, or luminol derivatives, or any other group which is detectable by Elisa or other means of detection. These compounds do not exist in natural DNA. The new compounds are useful as active monomers in a rapid and efficient procedure for the synthesis of DNA fragments and can be chemically incorporated in a DNA probe synthesis which allows total control over the extent and site of modification. |