| abstract |
A portable supplementary control system comprising a promoter/operator operably linked to a gene encoding the desired heterologous protein plus a gene encoding a compatible supplementary repressor protein operably linked to an inducible promotor, thus remedying any deficiency of repressor provided by the host's own genome, so that expression of a gene encoding a heterologous protein is repressed until a favourable point in the growth cycle of the host. The control system offers a method whereby heterologous protein production can be regulated by manipulation of the environment or of the medium. |