| abstract |
The invention relates to methods and agents for the immunological determination of enzymes which form an enzyme-inhibitor complex with an inhibitor, in particular for the determination of proteases. The method for the determination of enzymes with the aid of antibodies bound to a water-insoluble carrier against the enzyme to be determined is characterized in that enzymes which form an enzyme-inhibitor complex with an inhibitor are incubated with the bound antibodies, the sample solution is removed, incubated the washed-out, loaded with the enzyme-inhibitor complex with a coupling product, formed from an antibody reacting specifically with the inhibitor and a marker substance, the unbound coupling product removed and the concentration of the marker substance on the solid phase as a measure of the enzyme concentration to be determined |