http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EA-028341-B1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7a966a18aad148bb12c23fe66f2ecaa5 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-95 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-974 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6439 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6456 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6408 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-37 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 |
| filingDate | 2012-07-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_595df6b82328a1297d2279465edf39c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fce08ed7e41e7381651f335a5fd0a66b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70e81c5085552cc7021edb6622e56032 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05a6a59fe766cd8e7a401cc642558572 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9dcb7808aabe7da71ea959244885f408 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6eb845d823488b5bc385e4593dc9f01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a4ca42ac05dc0412994898b3a2d25a8 |
| publicationDate | 2017-11-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | EA-028341-B1 |
| titleOfInvention | METHOD FOR DETERMINING PROTEOLYTIC ENZYME ACTIVITY (VARIANTS), DEVICE FOR ITS IMPLEMENTATION AND METHOD OF DIAGNOSTICS |
| abstract | The present invention relates to medicine and biology and can be used, in particular, for diagnostic and research purposes to determine the characteristics of the blood coagulation system and its components. The present invention is the study of the spatial dynamics of coagulation factors in the growth of a fibrin clot and assessment of the role of coagulation factors in different phases of the blood coagulation process in a heterogeneous system, which allows to diagnose individual diseases and evaluate the activity of drugs that affect blood coagulation parameters. The problem is solved by creating a method for determining the spatial-temporal distribution of the activity of a proteolytic enzyme in a heterogeneous system, which provides an in vitro system that contains a sample of the test medium selected from the group consisting of blood plasma, whole blood, water, lymph, a colloidal solution, crystalloid solution or gel, and the proteolytic enzyme or its predecessor distributed in the sample, add a fluorogenic substrate to the in vitro system, followed by the release of labels when the substrate is cleaved by a proteolytic enzyme, a sample of the medium under study is illuminated at specified time points to excite the fluorescence signal of the label, the spatial distribution of the fluorescence signal of the label in the sample is recorded simultaneously with the illumination at specified time points, and is obtained from a set of spatial distributions of the fluorescent label signal for specified time points -time distribution of proteolytic enzyme activity by solving the inverse problem reaction-diffusion-convection type, taking into account the degree of label binding to the components of the medium under study. Chromogenic or luminescent substrates can be added as substrates. The proposed device for the implementation of this method. |
| priorityDate | 2011-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 83.