abstract |
A composition comprising- at least one mRNA comprising• at least one coding sequence encoding a spike protein (S) which is at least 95% identical to SEQ ID NO: 10,wherein the encoded spike protein (S ) a prefusion-stabilizing K986P and V987P mutation and further the mutation D614G,and• at least one heterologous untranslated region (UTR), and- at least one pharmaceutically acceptable carrier,wherein the at least one mRNA is complexed with one or more lipid(s), whereby forming lipid nanoparticles (LNP), the LNP comprising: (i) at least one cationic lipid; (ii) at least one neutral lipid; (iii) at least one steroid or steroid analogue; and (iv) at least one PEG-lipid, wherein (i) to (iv) in a molar ratio of about 20-60% cationic lipid, 5-25% neutral lipid, 25-55% sterol and 0.5-15% PEG -Lipid present. |