| abstract |
The invention provides a diluent for cryopreservation of chicken semen, which is characterized in that the diluent for cryopreservation of chicken semen comprises: pine needle antifreeze polypeptide, fructose, trehalose, potassium citrate monohydrate, magnesium acetate tetrahydrate, sodium glutamate , Tris (tris (trihydroxymethylaminomethane), reduced glutathione, oxidized glutathione and dimethylacetamide (DMA). The chicken semen cryopreservation diluent is a chicken semen cryopreservation diluent with easily available raw materials, convenient preparation, a fertilization rate of more than 80%, and higher DNA integrity. Compared with the relevant data of fresh chicken essence, the DNA integrity is close to that of fresh chicken essence, and its sperm motility and hatching rate are slightly lower than those of fresh chicken essence, which has broad application prospects. |