| abstract |
The present invention provides a method for preparing CD4-CD8-NKT cells and application thereof. The present invention uses skin fibroblasts and keratinocytes to replace thymic epithelial cells to establish thymic organoids, and domesticates CD4 - CD8 - NKT cells so that they only have immune regulation. It has no antitumor activity; blocked the secretion of TNF-α, made CD4 - CD8 - NKT secrete IFN-γ, and effectively inhibited the synthesis of IL-6 and TNF-α; the results showed that the above CD4 - CD8 - NKT cells Functional and secreted factor oriented, stable. |