| abstract |
The present invention provides an oligonucleotide synthesis catalyst and a preparation method thereof, and a method for synthesizing an oligonucleotide, wherein the oligonucleotide synthesis catalyst comprises tetrazoles, azole salts and pyridinium salts as main components At least one of the compounds of the class of compounds and imidazolium salts, and polar additives. The oligonucleotide synthesis catalyst of the present invention solves the problem of the current oligonucleotide synthesis process for complex (for example, the oligonucleotide chain length is too long, the GC content is high, and the repeat or continuous sequence is easy to produce secondary structures) The nucleotide sequence synthesis is difficult, and it is difficult to obtain the target oligonucleotide fragment or the target oligonucleotide fragment has many impurities and poor quality. When the oligonucleotide synthesis catalyst of the present invention is applied to oligonucleotide synthesis, the success rate of oligonucleotide synthesis with high GC content can be increased, and the quality of the oligonucleotide can be improved. |