http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114058641-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-415 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62 |
| filingDate | 2022-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114058641-B |
| titleOfInvention | Carrier system, application and method for degrading target protein through carrier system |
| abstract | The present invention relates to a vector system and use and a method for degrading a protein of interest therewith. The vector system comprises an expression vector containing a target gene sgRNA and Cas9, a fusion expression vector containing the target gene and an auxin induced degradation factor AID, and an auxin receptor-rice F-box transport inhibitory response protein 1 Os An expression vector for TIR1, and an expression vector containing a transposase; the expression vector containing the target genes sgRNA and Cas9 is an expression vector containing the target genes sgRNA and an expression vector containing Cas9 respectively, or an expression vector containing the target genes sgRNA and Cas9 simultaneously. The cells stably transfected by the vector system are treated by auxin, so that the problems of complicated knocking-in steps, low biallelic gene insertion efficiency and basic degradation of the traditional AID system are solved. |
| priorityDate | 2022-01-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 69.