| abstract |
The invention relates to the field of biomedicine, in particular to a primer combination, a kit and applications for triple PCR detection of TGEV, PEDV and PDCoV. The invention takes porcine epidemic diarrhea virus, porcine infectious gastroenteritis virus and porcine delta coronavirus as detection objects, and establishes a triple PCR detection method. By using Snapgene, DNAMAN, Primer5 and other software, a fragment with high homology among the three was found to design degenerate primers, which has important practical significance and clinical application value. |