| abstract |
The invention discloses a method for simultaneously detecting porcine getavirus (GETV), porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), porcine deltacoronavirus (PDCoV) and porcine group A A multiplex RT-PCR primer set for rotavirus (PoRV), the nucleotide sequences of the multiplex RT-PCR primer set are shown in SEQ ID NO:1-SEQ ID NO:10. The invention also discloses a multiple RT-PCR detection method for directly detecting GETV, PEDV, TGEV, PDCoV and PoRV from samples at one time by using multiple RT-PCR primer sets. Compared with the existing common RT-PCR, the detection method has strong specificity and high sensitivity, and can simultaneously identify GETV, PEDV, TGEV, PDCoV and PoRV five viruses, and the detection results are accurate and the detection efficiency is high. |