http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113005141-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44ae178fe5ea1fd193b8e5e1baf1ab3b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 |
| filingDate | 2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1bd7b8f1adcaa32c1cab5a38127e0916 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7863fc2bee3964fef6595c8ae09bbf1a |
| publicationDate | 2021-06-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-113005141-A |
| titleOfInvention | Gene editing tool composed of highly active mutant and preparation method and method for repairing the causative gene of congenital retinoschisis |
| abstract | The invention discloses an eaFnCpf1 gene editing tool composed of FnCpf1 mutants with high activity in human cells, a preparation method thereof, and a method for repairing the pathogenic gene of X-linked congenital retinoschisis, which is characterized by using human cells The highly active FnCpf1 mutant Q125R was obtained by in vitro directed evolution screening, and its expression vector is Addgene plasmids#69976; the guide for cutting the target DNA is crRNA, and its expression vector pJET‑U6‑crRNAs; the reporter cell line for gene editing is 293‑ SC1 and 293‑RS1; the homologous recombination repair template of mRS1 gene is a synthetic single-stranded deoxyribonucleotide. The acquisition of the high-activity FnCpf1 mutant includes establishing a FnCpf1 mutant library, screening mutant eaFnCpf1 with high editing activity and low off-target characteristics, and using eaFnCpf1 to edit endogenous genes. The highly active mutant eaFnCpf1 not only has high editing activity, but also can recognize a wider range of PAMs, which enriches the "skills" of gene editing tools to a certain extent, and helps to expand gene editing in the field of biomedicine Applications. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115968834-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111647618-A |
| priorityDate | 2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 80.