http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108486139-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_44ae178fe5ea1fd193b8e5e1baf1ab3b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2018-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_565f3326c8fdc0d2ddbf0f931267ed50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b825acb1500309243c1d105d63eea878 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6f736afc7b3d1243769b6b46e2d2378 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d85465eb982d02b6cc3e9b321e0695b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd90c94d338e2ee80152583e70d4a8bc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ae82902c949c32d5c030916e6f8f7ee |
| publicationDate | 2018-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-108486139-A |
| titleOfInvention | A kind of site-directed point mutation method and its application based on seamless clone technology |
| abstract | The present invention relates to a kind of site-directed point mutation method, kit and its applications based on seamless clone technology.Steps are as follows for the method：The forward and reverse mutant primer that 5 ' ends include complementary homology arm sequence is separately designed according to the sequence at mutational site, mutational site can be located at any position in homology arm；The forward and reverse primer that 5 ' ends include another complementary homology arm is separately designed in the non-mutated sites area of plasmid, the segment that two one end that PCR amplification goes out include mutational site need to have the difference in size of 500bp or more；For multisite mutation, the mutant primer of logarithm identical as number of sites is designed according to mutational site number；It is using homologous recombination enzyme that 25 one steps of segment are seamless spliced, build the cyclic plasmid with targeted mutagenesis site.The characteristics of relatively classical Overlap extension PCR method of the method, has fast and convenient, can realize single-point or multiple spot to being less than 40kb plasmids any position, continuously or discontinuously be mutated, and mutation efficiency is up to 100%. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108424907-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-110305861-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115927546-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111996188-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114703212-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-108424907-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113005141-A |
| priorityDate | 2018-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.