http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112779218-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a969dcb490d1270a2eede11532658c05 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-2306 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0642 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0787 |
| filingDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d7a38b6a4cbbf4da7c459f72cc3bd2f6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_530ad2e75df9663a1462b27c89435128 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f7328c7d554cdc9983b10b153d956232 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_47c524e728865a3de65092e0d45788fa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7af41424f0f3862ac7aea0dfd624cbf6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cca8bc15efd05dd5c8a9b03d49c0fbb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_36af37f57cbd5db840b56bfe5850b6f9 |
| publicationDate | 2021-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112779218-A |
| titleOfInvention | Medium and culture method for primary culture of tumor-infiltrating mast cells |
| abstract | The invention discloses a culture medium and a culture method for primary culture of tumor-infiltrating mast cells. The culture medium comprises the following components: 0.05-1v% PVA, 0.5-2v% ITSX, 80-120U/mL penicillin, 0.05‑1mg/mL streptomycin, 50‑60μM/L 2‑mercaptoethanol, 80‑120ng/mL SCF, 80‑120ng/mL IL‑6, and the balance is IMDM medium. The culture method includes the following steps: S1. Washing fresh tumor tissue, and then cutting it into small pieces of 2-4 mm 3 ; S2. Making the tissue piece obtained in S1 into a single cell suspension, then adding CD117 + immunomagnetic beads to incubate, washing the cells, Centrifuge and resuspend, and select mast cells by immunomagnetic bead sorting system; S3. Resuspend the mast cells obtained from S2 in the medium used for primary culture of tumor-infiltrating mast cells, and then transfer them to a well plate, and put them in a well plate. Culture in a 37°C, 5% CO 2 incubator, and perform half-volume medium changes 2-3 times a week. The efficiency of amplifying the mast cells by the method of the present invention is significantly improved, and the cost is significantly reduced at the same time. |
| priorityDate | 2021-01-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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