| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_50c84f5a031aef3f5b721b0272c3bd94 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-44
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-1353
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-45
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-237
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-415
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-39
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-155
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-119
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-067 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
| filingDate |
2018-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_74a8a7fa0193604addf643675ff81263 |
| publicationDate |
2020-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-111073843-A |
| titleOfInvention |
A method for maturation and expansion of hepatoid cells |
| abstract |
The present invention relates to a method for maturation and expansion of hepatoid cells. The steps include: step S1: in vitro culture of stem cells using an induction solution to induce mesenchymal stem cells to differentiate into hepatoid cells; step S2: targeting the hepatocytes through the spleen Inject into the FAH gene-deficient rat liver vector; Step S3: stimulate the autologous hepatocyte apoptosis of the FAH gene-deficient rat liver vector to promote the maturation and proliferation of hepatoid cells; Step S4: isolate and purify to obtain primary human hepatocytes. The invention induces human mesenchymal stem cells to form hepatocyte-like cells in vitro, and is injected into the liver of FAH gene-deficient rats through the spleen. And achieve rapid expansion of hepatocytes. Thus, the disadvantages of immature, small scale and high cost of hepatocytes obtained by the method of in vitro regeneration of hepatocytes are solved. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112779218-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112779218-B |
| priorityDate |
2018-10-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |