http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112538544-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-10 |
| filingDate | 2020-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112538544-B |
| titleOfInvention | Detection method and application of live bacteria of food-borne pathogenic bacteria standard strains with specific molecular targets |
| abstract | The present invention provides specific molecular targets for detecting food-borne pathogens, and the nucleotide sequences of the specific molecular targets are shown in SEQ ID NOs: 1-31. The present invention also provides a method for detecting viable food-borne pathogenic bacteria, comprising the following steps: step 1: adding a PMA dye solution to the sample to be tested; step 2: extracting bacterial DNA in the sample to be tested; step 3 : Use the bacterial DNA in step 2 as a template to perform real-time quantitative PCR detection; step 4: After the reaction is completed, calculate the food source in the sample by comparing the obtained fluorescence quantitative PCR amplification curve and Ct value with the established standard curve The number of viable sexually pathogenic bacteria. The detection method of the invention has the advantages of short detection time, does not need to go through the traditional culture and enrichment process, is not interfered by dead bacteria of the food-borne pathogenic bacteria standard strains in the sample, and can accurately detect and quantify the food-borne pathogenic bacteria standard strains in the sample. The specific number of bacteria contained. |
| priorityDate | 2020-12-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 20.