| abstract |
The invention provides a method for detecting food-borne pathogenic bacteria at high throughput. The method comprises the steps: performing polymerase chain reaction (PCR) amplification on a sample by using a plurality of universal primer pairs for pathogenic bacteria iconic DNA sequences so as to obtain the pathogenic bacteria iconic DNA sequences; contacting the pathogenic bacteria iconic DNA sequences with specific capture probes for the pathogenic bacteria iconic DNA sequences to obtain hybrid products with different detectable markers; and detecting signals of the detectable markers to determine the presence or the quantity of pathogenic bacteria in the sample. The invention also provides a kit for detecting the pathogenic bacteria. According to the method and the kit, the pathogenic bacteria can be accurately and quickly detected at high throughout, and have the advantages of easiness for operation, high specificity, high sensitivity and the like and have a wide application prospect. |