| abstract |
The invention discloses a method for preparing fish actin by a two-water phase method and its application. In the present invention, a two-phase system is obtained by mixing short-chain alcohol, salt and water; then, fish muscle myofibrillar protein or fish muscle myofibril protein solution is added to the two-phase system, and mixed to obtain a two-phase separation and extraction system , when the two-water-phase separation and extraction system appears to be layered again, the upper phase is taken to obtain a fish actin solution. The present invention also includes the step of quantifying fish actin with a capillary. The invention has high extraction rate and high purity, so that it can be used for fish actin production or fish actin standard product preparation. |