| abstract |
Label-free human galectin 13 and its preparation method and application relate to the field of protein preparation. The present invention first obtains the gene of human galectin 13, constructs an overexpression plasmid, induces the expression of human galectin 13 in Escherichia coli, the protein is purified by ammonium sulfate precipitation method, ion exchange column purification and gel filtration purification, Obtain label-free human galectin 13. The human galectin 13 prepared by the present invention has high purity, no label, exists in the human body naturally, and has certain safety for the human body. in addition. The present invention confirms through animal experiments that the prepared unlabeled human galectin 13 has the function of reducing the blood pressure of female rats and female rhesus monkeys, so the unlabeled human galectin 13 of the present invention can be used as a treatment for hypertension during pregnancy, Active ingredient in preeclampsia and HELLP syndrome. |