http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111662917-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e9192e7764cf16497c8ccfcd8e10e236 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-03001 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate | 2020-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52070e1e22e4e2b9096e3164f3be8f41 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a429875383d716fbf6f6ff0e4fe7276 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_601ce4c6c96f7061d44391172bf4f3ba http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_23e18549e7dbcec05f4acc0b151f6eca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00f73214710e052b5e0f980bc0bcb2ee |
| publicationDate | 2020-09-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111662917-A |
| titleOfInvention | A kind of high specific activity alkaline phosphatase engineering bacteria, engineering bacteria construction and alkaline phosphatase purification method |
| abstract | The invention discloses an alkaline phosphatase engineering bacterium with high specific activity, a method for constructing the engineering bacterium and an alkaline phosphatase purification method, and belongs to the technical field of biological engineering. In the present invention, the alkaline phosphatase gene of Cobetia marina is optimized by codons, and the optimized alkaline phosphatase gene is used to construct an engineering bacterium, and the preservation number of the engineering bacterium is CGMCC NO.18926. When expressed by the genetically engineered bacteria constructed in the present invention, the fermentation amount can reach 10 mg (1 L fermentation broth). The invention also innovates the purification method of the protein. Using the purification method of the invention, an enzyme protein with a final purity of more than 95% can be obtained, and the specific activity can reach 12000U/mg, which is compared with the existing international common alkaline phosphatase. , is a highly active alkaline phosphatase, which lays the groundwork for further industrialization and provides an effective method for industrial large-scale production of alkaline phosphatase. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113564185-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113564185-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-113621550-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112341533-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114196688-A |
| priorityDate | 2020-06-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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