| abstract |
The invention relates to an anti-SARS octapeptide liquid-phase synthesis method using tris(4-diphenylphosphonooxybenzoylphenyl) phosphate TDPBP and its derivatives as peptide synthesis small molecule carriers. Using tris(4-diphenylphosphonooxybenzoylphenyl) phosphate TDPBP derivatives to replace solid-phase resin, under the action of coupling dehydrating agent, with N-terminal and side chain protected arginine C End connection; after separation and purification, remove N-terminal Fmoc; De-Fmoc reaction is performed to prepare the precursor of the anti-SARS octapeptide; the side chain is deprotected and the carrier is cut off to obtain the solid of the anti-SARS octapeptide. Compared with the existing synthesis methods, the present invention is simple, fast, economical and efficient to synthesize and prepare anti-SARS octapeptides on a large scale, and the auxiliary groups derived from TDPBP are easy to be recovered and recycled after regeneration, thereby reducing waste of raw materials and waste. pollution, cost savings, and environmental protection. |