http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-112147185-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2440-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-485 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-558 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6818 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2019-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-112147185-B |
| titleOfInvention | A method for controlling the speed of polypeptide passing through nanopore and its application |
| abstract | The present invention provides a method for controlling the speed of a polypeptide passing through a nanopore and its application in the determination of the amino acid sequence of a polypeptide, in particular, connecting a nucleic acid to a polypeptide to obtain a nucleic acid-polypeptide linker, and applying a nucleic acid-polypeptide linker in the presence of a nuclease. The voltage across the nanopore makes the ligation product pass through the nanopore, and the nuclease controls the movement of the nucleic acid and then the movement of the linked polypeptide in the nanopore, so as to control the speed of the polypeptide passing through the nanopore. While controlling the speed of the polypeptide, the present invention reads the nanopore current signal during the process of the polypeptide passing through the nanopore to obtain the electrical signal waveform of the polypeptide, and the electrical signal waveform can be further used to obtain the amino acid sequence of the polypeptide, or used for The identification of the polypeptide, or a portion thereof, can also be used to construct a library of electrical signal waveforms of the polypeptide. |
| priorityDate | 2019-06-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 128.