http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111793606-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2810-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-06 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0656 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-495 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 |
| filingDate | 2019-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-04-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111793606-B |
| titleOfInvention | Method for improving CRISPR/Cas9-mediated homologous repair efficiency |
| abstract | The invention relates to the technical field of genetic engineering, in particular to a method for improving CRISPR/Cas9-mediated homologous repair efficiency. The invention provides a cell culture solution for culturing gene editing cells after transfection, which comprises a basic culture solution and a p53 protein small molecule agonist; the p53 protein small molecule agonist comprises one or more of RITA, CTX1 and Nutlin-3. The invention also provides a method for improving homologous repair efficiency of gene editing by using the culture solution. By adding the micromolecule agonist of the p53 protein into the cell culture solution for culturing the gene editing cells after transfection, the homologous repair efficiency of the gene editing process is greatly improved, the gene editing efficiency is further improved, the screening workload of positive cells is effectively reduced, and the method has the advantages of simplicity and convenience in operation, low cost and the like, and has a wide application prospect. |
| priorityDate | 2019-04-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.