http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111748564-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07D403-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-165 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-65 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D403-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P17-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-65 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 |
| filingDate | 2020-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111748564-B |
| titleOfInvention | Genetically modified purple bacteriocin biosynthesis gene cluster, recombinant expression vector, engineered bacteria and their applications |
| abstract | The invention discloses genetically modified violetin biosynthesis gene clusters, recombinant expression vectors, engineering bacteria and applications thereof. The ribosome binding sites of violacein biosynthesis gene clusters are modified by site-directed mutation and codon deletion mutation , to promote the efficient translation of the violetin synthesis protein, and obtain a modified gene cluster with significantly improved production potential; by introducing the above-mentioned modified gene cluster or a recombinant vector containing the modified gene cluster into the host bacteria, an engineered bacteria with high production of violetobactin was obtained; The fermentation conditions of the engineered bacteria were further optimized and a method for the separation and purification of violetobactin was provided. The genetically engineered strain constructed by the present invention can not only significantly improve the yield of violetobactin, but also can efficiently produce violetobactin metabolism under the temperature condition of 25-37 ℃, which is different from the conventional low-temperature fermentation of violetobactin at 20°C or 25°C. The product solves the problem of high cooling cost in the fermentation process, and is suitable for large-scale production. |
| priorityDate | 2020-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 218.