http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111560478-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-165 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2020-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2020-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2020-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-111560478-B |
| titleOfInvention | Kit for detecting novel coronavirus by combining reverse transcription PCR with Sanger sequencing in one-step method |
| abstract | The invention discloses a kit for detecting novel coronavirus by combining reverse transcription PCR with Sanger sequencing in a one-step method. The invention provides a primer combination capable of specifically amplifying ORF1a, ORF1ab and 3 structural protein (S protein, E protein and N protein) genes of a novel coronavirus COVID-19, and a kit for detecting the novel coronavirus COVID-19 is constructed by utilizing the primer combination and combining one-step reverse transcription PCR and Sanger sequencing, so that the kit realizes the one-step completion of RNA reverse transcription and PCR amplification, simplifies the operation steps and shortens the detection time; in addition, the detection accuracy, sensitivity and specificity of the kit are all up to 100%, and the minimum detection amount and detection repeatability are verified to pass; therefore, the kit has important clinical significance when being applied to the detection of the novel coronavirus COVID-19. |
| priorityDate | 2020-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 301.