| abstract |
The invention provides a primer, a probe and a kit for detecting novel coronavirus. The invention designs specific primers and probes according to ORF1ab gene, E gene, N gene, S gene and M gene of the novel coronavirus 2019-nCoV, and is used for identifying and detecting the novel coronavirus. The specific primer/probe and the kit for detecting 2019-nCoV have the advantages of high sensitivity, precision and accuracy, good specificity, good stability, less template amount required by detection and objective and accurate detection result. The five-target design can better overcome false negative caused by variation in the process of passage of RNA viruses, effectively improve the positive detection rate and avoid the condition of missed detection as far as possible. Has high clinical application value. |